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課程名稱 |
光學方法在生物研究之應用
OPTICAL METHODS FOR BIOLOGICAL RESEARCH |
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開課學期 |
98-1 |
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授課對象 |
理學院 化學系 |
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授課教師 |
張大釗 |
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課號 |
Chem3029 |
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課程識別碼 |
203 35130 |
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班次 |
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學分 |
3 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期四3,4,@(10:20~) |
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上課地點 |
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備註 |
本課程中文授課,使用英文教科書。原分所311.詳選課須知.限大三大四.外系所學生需經授課教師同意.與李弘文合開
限學士班學生 且 限學士班三年級以上 且 限本系所學生(含輔系、雙修生)
總人數上限：20人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/981optical4bio |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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課程概述 |
Goal of this course is to show how optical methods can make a difference in our understanding of significant biology problems. Topics such as ligand-DNA interaction, cancer biology, cell tracking and molecular motors are introduced. We hope to introduce the significant biological principles involved and the range of optical methods that can be employed to produce new knowledge about these topics at a fundamental level. |
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課程目標 |
Instructors:
Sheng-Hsien Lin (IAMS, Sinica)
Yuen-Ron Shen (Berkeley, Physics)
Edward S. Yeung (Iowa State, Chemistry)
Ta-Chau Chang (IAMS, Sinica, coordinator)
Hung-Wen Li (NTU, Chemistry, co-coordinator)
(1). Introduction to optical methods (Chang and Li) 12-18 hrs
- basic spectroscopy (introduction of laser, group theory and vibrational spectroscopy), various spectroscopic methods
- basic microscopy (introduction to various imaging, tracking methods and their applications in biological sciences)
(2). Non-linear spectroscopy (Shen) 4 hrs
(3). Novel spectroscopy and microscopy approaches to Biology (Yeung) 4 hrs
(4). Theory (Lin) 4 hrs
(5). Research projects (8 half-days in the research lab)
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課程要求 |
Grading scheme:
15% homework
35% final
50% research project
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
Research projects: As part of the course, students are required to engage a research project, in a group of 5 people, which uses the materials learned from this course to address a biological question. The project is a required part of the course, and will take 50% of the final grade.
(i). Single molecule method
single molecule TPM method/magnetic tweezers technique to study protein-DNA interaction
(ii). Spectroscopy Combination
Absorption, fluorescence and CD spectroscopy in studying DNA topology
(iii). Raman, CARS and SERS spectroscopy/microscopy
Non-linear spectroscopy for imaging
(iv). fluorescence lifetime, FLIM, FRET methods
Fluorescence-based method for study ligand-DNA interaction
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參考書目 |
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評量方式
(僅供參考) |
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週次 |
日期 |
單元主題 |
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第1週 |
9/17 |
Small molecules in cancer research |
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第2週 |
9/24 |
Group Theory |
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第3週 |
10/01 |
Vibrational spectra:IR,Raman,Hole burning |
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第6週 |
10/22 |
LASER |
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第7週 |
10/29 |
optical microscopy (HWLi) |
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第8週 |
11/05 |
single molecule thinking (HWLi) |
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第10週 |
11/19 |
microscopy method (HWLi) |
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第17週 |
1/07 |
Project Presentation |
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